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Aplysia neuron after axotomy

Supplemental videos for Journal of Cell Biology (JCB)

These files are in AVI format. 

Videos 1, and 2. The formation of MT-based vesicle traps after axotomy within the proximal cut end. The videos illustrate the sequences depicted by Fig. 3. Video 1 shows the sequence of EB3-GFP dissipation, reformation and reorientation to form the plus- and minus-end traps. The accumulations of organelles labeled by RH237 in two locations that spatially correspond to the plus- and minus-end traps are shown in Video 2. With time, some of the plus ends of the MTs at the GCOC polymerize centrifugally pointing their plus end tips towards the plasma membrane of the extending GC's lamellipodium. This is associated with reduction of the RH237 labeled vesicles signal within the GCOC. The videos are composed of 181 images, taken at intervals of 6 seconds. The frames are shown at a rate of 10/s.  

Video 1

Video 2

Videos 3, 4, 5 and 6. The formations of a minus-end vesicle trap after axotomy within the distal cut end.  The videos illustrate the sequences depicted by Fig. 4 and show the merged images of EB3-GFP (green) and RH237 (red) in the distal segment at different times before, during and post axotomy. Video 3- before and during axotomy, Video 4- 9 min. , Video 5   18 min. and Video 6- 61 min. post axotomy. In the distal segment a minus-end trap is form at the GCOC. This trap concentrates RH237 labeled vesicles. With time, some of the plus ends of the MTs at the GCOC polymerize centrifugally pointing their plus end tips towards the plasma membrane of the extending GC's lamellipodium. This is associated with elevation of the RH237 labeled vesicles signal within the GCOC. Those videos compose of 82, 59, 60 and 29 images taken at intervals of 7 seconds. The frames are shown at a rate of 10/s.  

Video 3 

Video 4

Video 5

Video 6

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