Supplement movies for the
Journal of Comparative Neurology- 2007
These files are in AVI format.
Movies 1 and 2. The
formations of a minus-end vesicle trap after axotomy within the distal cut
The movies illustrate the sequences depicted by Fig. 4 and show the
merged images of EB3-GFP (green) and RH237 (red) in the distal segment before,
during and after axotomy. Movie -1, before and during axotomy, Movie -2, 18
minutes post axotomy. The formed trap concentrates RH237 labeled vesicles. The
movies are composed of 82 and 60 images correspondingly, taken at intervals of 7
seconds. The frames are shown at a rate of 10/s.
Movie 3. The MTs polymerize
centrifugally from the minus end trap formed at the cut end of the distal axon. A
merged image of EB3-GFP (green)
and SR101 (red) in the distal segment 3 hours post axotomy.
Whereas the MTs of the dGCOC polymerize laterally to form finger-like
protrusions, only a negligible fraction of the trapped SR101-labeled vesicles
translocate centrifugally. Instead, the labeled vesicles continue to accumulate
within the dGCOC. The movie is composed
of 15 images, taken at intervals of 18 seconds. The frames are shown at a rate
Supplementary Movies 4, 5
and 6. Inhibition
of the retrograde vesicles transport doses not interfere with the formation of
the minus end trap. The shown
images were taken from a B
neuron in which endocytotic vesicles are labeled by SR101 and the plus-end
tips of the MTs are labeled by EB3-GFP.
Movie-4, Control, demonstrating the retrograde transport of SR101
labeled vesicles. Movie-5 was taken after a 3 hours incubation period in
EHNA. Note the dramatic reduction in the retrograde transport of SR 101 labeled
vesicles. Movies 4 and 5 are composed of
20 images taken at intervals of 7 seconds. The frames are shown at a rate of
Movie-6 was taken 22 minutes after axotomy. Although most of the
SR101 labeled vesicles are stationary in the presence of EHNA, the MT
based minus-end trap was formed. This movie is composed of 30 images taken
at interval of 7 second. The
frames are shown at a rate of 10/s.